hascs pt-5006 Search Results


porous gelma construct  (ATCC)
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ATCC porous gelma construct
Fig. 1 Schematics of an in situ handheld printing system. a A schematic of an in situ handheld printing system to obtain microbubbles. b Optical and SEM images of a <t>porous</t> <t>hASCs-laden</t> <t>GelMa</t> structure located in a cylindrical mold. Scale bar, 200 µm (b).
Porous Gelma Construct, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1 Schematics of an in situ handheld printing system. a A schematic of an in situ handheld printing system to obtain microbubbles. b Optical and SEM images of a porous hASCs-laden GelMa structure located in a cylindrical mold. Scale bar, 200 µm (b).

Journal: NPJ Regenerative medicine

Article Title: The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.

doi: 10.1038/s41536-023-00307-1

Figure Lengend Snippet: Fig. 1 Schematics of an in situ handheld printing system. a A schematic of an in situ handheld printing system to obtain microbubbles. b Optical and SEM images of a porous hASCs-laden GelMa structure located in a cylindrical mold. Scale bar, 200 µm (b).

Article Snippet: The cell-laden porous GelMa construct [hASCs, PT-5006; Lonza, Basel, Switzerland, or C2C12, CRL-1772; ATCC, Manassas, VA, USA (1 × 107 cells/mL)] was fabricated in a polydimethylsiloxane (PDMS) mold (diameter: 8 mm, height: 10 mm).

Techniques: In Situ

Fig. 2 Bubble formation for various rheological properties of bioink. a Complex viscosity (Pa·s) of various GelMa concentrations (5, 10, and 15% w/v) and (b) the air-bubbles formed at two typical temperatures [(i) 15 °C and (ii) 33 °C]. c Optical and live (green)/dead (red) images of air bubbles formed with various GelMa concentrations (Vtotal: total volume including air bubbles and solution and Vfoam: volume of air bubbles). d Pore size, (e) pore size coefficient of variation, defined with standard deviation divided by average value, (f) foamability (Vfoam/Vtotal), and (g) cell viability of the porous GelMa structures for various GelMa concentrations. All data are presented as mean ± standard deviation and the p- values were determined by one-way ANOVA followed by Tukey’s test (d, e, f, g) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 2 mm (b); 1 mm (b-optical images); 200 µm (b-microscopic images and live/dead images).

Journal: NPJ Regenerative medicine

Article Title: The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.

doi: 10.1038/s41536-023-00307-1

Figure Lengend Snippet: Fig. 2 Bubble formation for various rheological properties of bioink. a Complex viscosity (Pa·s) of various GelMa concentrations (5, 10, and 15% w/v) and (b) the air-bubbles formed at two typical temperatures [(i) 15 °C and (ii) 33 °C]. c Optical and live (green)/dead (red) images of air bubbles formed with various GelMa concentrations (Vtotal: total volume including air bubbles and solution and Vfoam: volume of air bubbles). d Pore size, (e) pore size coefficient of variation, defined with standard deviation divided by average value, (f) foamability (Vfoam/Vtotal), and (g) cell viability of the porous GelMa structures for various GelMa concentrations. All data are presented as mean ± standard deviation and the p- values were determined by one-way ANOVA followed by Tukey’s test (d, e, f, g) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 2 mm (b); 1 mm (b-optical images); 200 µm (b-microscopic images and live/dead images).

Article Snippet: The cell-laden porous GelMa construct [hASCs, PT-5006; Lonza, Basel, Switzerland, or C2C12, CRL-1772; ATCC, Manassas, VA, USA (1 × 107 cells/mL)] was fabricated in a polydimethylsiloxane (PDMS) mold (diameter: 8 mm, height: 10 mm).

Techniques: Viscosity, Pore Size, Standard Deviation

Fig. 4 Bubble formation for various mesh filter conditions. a Schematic of a handheld printing system with various sizes of mesh filter. b Optical images of the air bubbles formed with various sizes and numbers of the mesh filter [(i) without filter and (ii) with filters]. c Fabricated pore size and (d) foamability of the porous GelMa structures for various filter sizes and filter numbers. All data are presented as mean ± standard deviation and the p-values were determined by one-way ANOVA followed by Tukey’s test (c, d) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 500 µm (a, b).

Journal: NPJ Regenerative medicine

Article Title: The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.

doi: 10.1038/s41536-023-00307-1

Figure Lengend Snippet: Fig. 4 Bubble formation for various mesh filter conditions. a Schematic of a handheld printing system with various sizes of mesh filter. b Optical images of the air bubbles formed with various sizes and numbers of the mesh filter [(i) without filter and (ii) with filters]. c Fabricated pore size and (d) foamability of the porous GelMa structures for various filter sizes and filter numbers. All data are presented as mean ± standard deviation and the p-values were determined by one-way ANOVA followed by Tukey’s test (c, d) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 500 µm (a, b).

Article Snippet: The cell-laden porous GelMa construct [hASCs, PT-5006; Lonza, Basel, Switzerland, or C2C12, CRL-1772; ATCC, Manassas, VA, USA (1 × 107 cells/mL)] was fabricated in a polydimethylsiloxane (PDMS) mold (diameter: 8 mm, height: 10 mm).

Techniques: Pore Size, Standard Deviation

Fig. 5 Bubble formation for various volume ratios of bioink and air. a Optical images (red dot-line = Vfoam and white dot-line = Vtotal), (b) foamability, and (c) pore size of the porous GelMa structures fabricated with various volume ratios of bioink and air. All data are presented as mean ± standard deviation and the p-values were determined by one-way ANOVA followed by Tukey’s test (b, c) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 1 mm (b, a-optical images); 500 µm (b-microscopic images).

Journal: NPJ Regenerative medicine

Article Title: The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.

doi: 10.1038/s41536-023-00307-1

Figure Lengend Snippet: Fig. 5 Bubble formation for various volume ratios of bioink and air. a Optical images (red dot-line = Vfoam and white dot-line = Vtotal), (b) foamability, and (c) pore size of the porous GelMa structures fabricated with various volume ratios of bioink and air. All data are presented as mean ± standard deviation and the p-values were determined by one-way ANOVA followed by Tukey’s test (b, c) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 1 mm (b, a-optical images); 500 µm (b-microscopic images).

Article Snippet: The cell-laden porous GelMa construct [hASCs, PT-5006; Lonza, Basel, Switzerland, or C2C12, CRL-1772; ATCC, Manassas, VA, USA (1 × 107 cells/mL)] was fabricated in a polydimethylsiloxane (PDMS) mold (diameter: 8 mm, height: 10 mm).

Techniques: Pore Size, Standard Deviation

Fig. 6 Bubble formation for various flow rates. a Optical images and live (green)/dead (red) images of porous GelMa constructs fabricated using various flow rates (3~12 mL/s). Measured (b) pore size, (c) foamability, and (d) cell viability. All data are presented as mean ± standard deviation and the p-values were determined by one-way ANOVA followed by Tukey’s test (b, c, d) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 1 mm (a-optical images); 200 µm (a-microscopic images and live/dead images).

Journal: NPJ Regenerative medicine

Article Title: The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.

doi: 10.1038/s41536-023-00307-1

Figure Lengend Snippet: Fig. 6 Bubble formation for various flow rates. a Optical images and live (green)/dead (red) images of porous GelMa constructs fabricated using various flow rates (3~12 mL/s). Measured (b) pore size, (c) foamability, and (d) cell viability. All data are presented as mean ± standard deviation and the p-values were determined by one-way ANOVA followed by Tukey’s test (b, c, d) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 1 mm (a-optical images); 200 µm (a-microscopic images and live/dead images).

Article Snippet: The cell-laden porous GelMa construct [hASCs, PT-5006; Lonza, Basel, Switzerland, or C2C12, CRL-1772; ATCC, Manassas, VA, USA (1 × 107 cells/mL)] was fabricated in a polydimethylsiloxane (PDMS) mold (diameter: 8 mm, height: 10 mm).

Techniques: Construct, Pore Size, Standard Deviation

Fig. 7 In vitro study of the porous GelMa structure compared to bulk hydrogel. a Optical images of the normal non-porous bioink (control) and porous bioink (experimental). b Volume fraction of the bioinks consisting of GelMa, PBS, and air of the fabricated structures. c Porosity and density of the structures. d Schematic of the cell containing mold. e MTT assay of hASC proliferation in both structures at 1, 3, and 7 days. f Live/dead images at 7 days and (g) cell viability. h Dapi (blue)/phalloidin (red) images and (i) F-actin area. j Dapi (blue)/MHC (green) images of the top, middle, and bottom region in the control and experimental group and (k) positive index of MHC. All data are presented as mean ± standard deviation and the p-values were determined by student’s t-test (c, e, g, i, k) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 1 mm (a-optical images); 500 µm (b-microscopic images); 200 µm (f, h); 100 µm (j).

Journal: NPJ Regenerative medicine

Article Title: The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.

doi: 10.1038/s41536-023-00307-1

Figure Lengend Snippet: Fig. 7 In vitro study of the porous GelMa structure compared to bulk hydrogel. a Optical images of the normal non-porous bioink (control) and porous bioink (experimental). b Volume fraction of the bioinks consisting of GelMa, PBS, and air of the fabricated structures. c Porosity and density of the structures. d Schematic of the cell containing mold. e MTT assay of hASC proliferation in both structures at 1, 3, and 7 days. f Live/dead images at 7 days and (g) cell viability. h Dapi (blue)/phalloidin (red) images and (i) F-actin area. j Dapi (blue)/MHC (green) images of the top, middle, and bottom region in the control and experimental group and (k) positive index of MHC. All data are presented as mean ± standard deviation and the p-values were determined by student’s t-test (c, e, g, i, k) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005). Scale bar, 1 mm (a-optical images); 500 µm (b-microscopic images); 200 µm (f, h); 100 µm (j).

Article Snippet: The cell-laden porous GelMa construct [hASCs, PT-5006; Lonza, Basel, Switzerland, or C2C12, CRL-1772; ATCC, Manassas, VA, USA (1 × 107 cells/mL)] was fabricated in a polydimethylsiloxane (PDMS) mold (diameter: 8 mm, height: 10 mm).

Techniques: In Vitro, Control, MTT Assay, Standard Deviation

Fig. 9 In vivo study of the porous GelMa structure. Schematics demonstrating the fabrication process and parameters of (a) the GelMa foam and (b) the normally bioprinted structure. c Optical images of the VML models of sham, defect, acellular foam, hASC-printed, and hASC-foam on day 0 and day 28. d Grip strength for 1 to 4 weeks and (e) latency to fall and (f) muscle weight at 4 weeks after implantation. All data are presented as mean ± standard deviation and the p-values were determined by one-way ANOVA followed by Tukey’s test (d, e, f) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005).

Journal: NPJ Regenerative medicine

Article Title: The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.

doi: 10.1038/s41536-023-00307-1

Figure Lengend Snippet: Fig. 9 In vivo study of the porous GelMa structure. Schematics demonstrating the fabrication process and parameters of (a) the GelMa foam and (b) the normally bioprinted structure. c Optical images of the VML models of sham, defect, acellular foam, hASC-printed, and hASC-foam on day 0 and day 28. d Grip strength for 1 to 4 weeks and (e) latency to fall and (f) muscle weight at 4 weeks after implantation. All data are presented as mean ± standard deviation and the p-values were determined by one-way ANOVA followed by Tukey’s test (d, e, f) (NS = statistical nonsignificance, *p < 0.05, **p < 0.005, and ***p < 0.0005).

Article Snippet: The cell-laden porous GelMa construct [hASCs, PT-5006; Lonza, Basel, Switzerland, or C2C12, CRL-1772; ATCC, Manassas, VA, USA (1 × 107 cells/mL)] was fabricated in a polydimethylsiloxane (PDMS) mold (diameter: 8 mm, height: 10 mm).

Techniques: In Vivo, Standard Deviation